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. 2016 Dec 26;7:649. doi: 10.3389/fimmu.2016.00649

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Copyright © 2016 Chu, Zhu, You, Zhang, Zheng, Cai, Zhou, Wang, Sun, Yang, Zhang, Wang, Nie, Zhu and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The hS9-Fab03 inhibits lipopolysaccharide (LPS)-induced inflammatory cytokines production in THP-1-derived macrophages. PMA-stimulated THP-1-differentiated macrophages in 24 wells were pre-incubated with 5 µg/ml hS9-Fab03 for 2 h, then stimulated with 1 µg/ml LPS. Anthrax chimeric Fab antibody was used as the negative control. The mRNA expression levels of IFN-β (A), TNF-α (B), IL-6 (C), IL-1β (D), IL-10 (E), and IL-8 (F) were detected by Q-PCR. Experiments were performed in triplicate. Data are shown as mean ± SD (n = 3, N.S., not significant, *p < 0.05, **p < 0.01, ***p < 0.001 compared to negative control).