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. 2016 Dec 26;7:649. doi: 10.3389/fimmu.2016.00649

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Copyright © 2016 Chu, Zhu, You, Zhang, Zheng, Cai, Zhou, Wang, Sun, Yang, Zhang, Wang, Nie, Zhu and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Western blot analysis for inhibition of lipopolysaccharide (LPS)-induced NF-κB, MAPKs, and IRF-3 activation by hS9-Fab03. Cells were pretreated with 5 µg/ml of the hS9-Fab03 for 2 h and further incubated in presence or absence of LPS (1 µg/ml). After immunoblotting, the phosphorylation levels of p65, JNK1/2, p38, and IRF-3 were identified using phosphor-specific antibodies. Total p38 and GAPDH were used to ensure equal loading. L, LPS; A, hS9-Fab03. Data are shown as mean ± SD (n = 3, N.S., not significant, *p < 0.05, **p < 0.01, ***p < 0.001 compared to negative control).