Figure 4.

Effect of Periplocin on proinflammatory cytokine expression in splenocytes from Freund's complete adjuvant Sprague Dawley rats. Splenocytes were prepared as described under Methods. Splenocytes were cultured in the presence or absence of Periplocin or LPS for indicated time. The total mRNA was prepared for RT-PCR analysis. (a) Chemical structure of Periplocin, (b) RT-PCR analysis of inflammatory cytokine genes and related transcription factors, and relative amounts of each cytokine gene level were determined by densitometric analysis. L: LPS; P: Periplocin; ●: LPS only; ○: LPS + P; ▲: P only.