Table 1.
Primers designed for the amplification of trehalose operon genes in C. perfringens along with the upstream regions.
| Name | Gene | Sequence | Location | Size |
|---|---|---|---|---|
| CPF_0539F | Sigma-70 factor | TGAGTTCCAAAATAGACAAGAT | 640794–640815 | 22-mer |
| CPF_0539R | Sigma-70 factor | CCCCATAAGCCAAGGTG | 642208–642224 | 17-mer |
| CPF_0540F | Hypothetical protein | AATGGGGTAGGAATAACTTTGTCA | 642326–642349 | 24-mer |
| CPF_0540R | Hypothetical protein | CTTTTCCGCCTATGTACTCTGTTA | 643493–643516 | 24-mer |
| 1985F | CPF_0540 upstream | ACAAGCTTTAGGTGAGGTTT | 641985–642004 | 20-mer |
| tre373F | treB | TGATATGAATAAGGAGGGTGTTGG | 642793–642816 | 24-mer |
| tre1027F | treB | TATGGAGGGAAAAAGATGAGTAAG | 643447–643470 | 24-mer |
| tre2172R | treB | TGCTGGTATTGATATTTGTTTTTC | 644569–644592 | 24-mer |
| tre1920F | treB | TATAGTGGCAGGCTTAACATCATC | 644340–644363 | 24-mer |
| tre3128R | treC | CTCTCCTAGCTCCTCAACATACTC | 645525–645548 | 24-mer |
| tre3090F | treC | GGAGGGAATGCTTGGGAGTA | 645510–645529 | 20-mer |
| tre3777R | treC | AACCTGGATTTGTCATTC | 646180–646197 | 18-mer |
| tre3688F | treC | CTTCAAAAATGCTAGCGACTTCAA | 646108–646131 | 24-mer |
| tre4667R | treC-treR | TGCTTCAAAAATTCCACTATCTA | 647065–647087 | 23-mer |
| tre4406F | treR | AAATTAAGAAGGGGTTGTAT | 646826–646845 | 20-mer |
| tre5283R | treR | ACTTAAATTTATCTGGTCTATGTC | 647680–647703 | 24-mer |
| treRR | treR | CTTTAATAGCTGGTGATAGTT | 648367–648387 | 21-mer |