Fig. 4.

Deletion of PGC-1s decreases ETC subunit gene expression but not protein expression. A: PGC-1 family mRNA expression. B: ETC subunit mRNA expression. C: representative Western blot of ETC protein expression. D: densitometry quantification of ETC protein expression normalized to Ponceau S (Ponc) staining from gastrocnemius (GAS) muscle of iMyo-PGC-1DKO and control littermates, exercised and sedentary. E: percent change in complex activity with exercise from sedentary animals. Data are presented as means ± SE; n = 5 per group; *P < 0.05 compared with sedentary control; #P < 0.05 compared with sedentary iMyo-PGC-1DKO animals as determined by 2-way ANOVA with pairwise comparisons (Tukey adjustment) for A, B–D.