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. 2016 Dec 26;7:1931. doi: 10.3389/fpls.2016.01931

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Copyright © 2016 Sinha, Pospíšil, Maheshwari and Eudes.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effect of Bcl-2△21 and Ac-DEVD-CHO on isolated microspore culture in wheat cultivar Fielder. Bars 20 μM. (A–C) Isolated microspore culture after 4 weeks of inductive stress treatment and incubation, the figure showing large developing embryos; (A) control; (B) Bcl-2△21 and (C) Ac-DEVD-CHO treated microspore culture with supplemented ovaries; (D) the percent viability of microspore cells treated or not with Bcl-2△21 and Ac-DEVD-CHO; (E) comparison of caspase-3-like activity in control, Bcl-2△21 and Ac-DEVD-CHO treated microspore culture. The bars denote the mean standard error of at least five independent experiments. The asterisk (^∗) and different letters indicate significant differences at P < 0.05 according to Tukey’s test.