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. 2016 Dec 26;7:1931. doi: 10.3389/fpls.2016.01931

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Copyright © 2016 Sinha, Pospíšil, Maheshwari and Eudes.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Scavenging effect of Bcl-2△21 and Ac-DEVD-CHO on hydroxyl radical formation. Detection of DMPO-OH adduct EPR signal in the total protein isolated from the wheat microspore culture in the presence of (A) Bcl-2△21 and (B) Ac-DEVD-CHO. H₂O₂-induced DMPO adduct spectra were measured in the presence of 50 mM DMPO and 40 mM Mes (pH 7.2). An amount of 5 ug of total protein was used for each measurement. Letter (a) control, (b) H₂O₂, and (c) H₂O₂ + mannitol denotes trace a, trace b, and trace c, respectively. All other conditions were as mentioned in Figure 1.