Figure 5. siRNA suppression of treacle and effect on p53.

(“NS” = transfection with non-specific control siRNA; “Treacle” = transfection with treacle-specific siRNA). a) Treacle-specific siRNA reduced treacle expression at 24 hrs and abolished expression at 48 hrs, but had no effect on expression of p53 or the β-actin control. b) The response of p53 to H₂O₂ was the same for cells transfected with treacle-specific and control siRNA. Neither siRNA nor H₂O₂ affected expression of the β-actin control. The data were representative of three independent experiments.