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. Author manuscript; available in PMC: 2017 Dec 1.
Published in final edited form as: Biofabrication. 2016 Dec 1;9(1):015002. doi: 10.1088/1758-5090/9/1/015002

Figure 7.

Figure 7

Resized cluster loaded into 3D-printed device are resistant to hypoxia-induced death. (a–c) Schematics illustrating test set up for (a) free floating large clusters, (b) large clusters loaded into device, and (c) small clusters loaded into device. (d–f) Representative images of clusters stained with a fluorescent viability dye after 48 hr of culture at 40 mmHg pO2,gas taken with the same exposure. The top images are composites of both the live (green) and dead (red) stains, middle images just dead stain, and bottom images just green stain. Panel (d) was cultured as indicated in panel (a), (e) as in (b), and (f) as in (c). Scale bar=200 μm (g) Histological section of SC-β cell clusters cultured as in (b) stained with a TUNEL assay (red) and with DAPI (blue). Scale bar=50 μm (h) Quantitation of TUNEL+ cells from histological sections. *p<0.01 (two-sided unpaired t-test).