Figure 8.

3D-printed devices loaded with SC-β cells (5x10⁶ cells per mouse) are functional after transplantation into mice. (a) In vivo glucose stimulated insulin secretion assays of mice 2 wk (n=6 for large clusters and n=7 for small clusters) and 12 wk (n=3 for small clusters) after transplantation. Mice were fasted overnight and serum collected before (0 min) and 30 min after an intraperitoneal injection of glucose. Human insulin was quantified from this serum using ELISA. (b) Image demonstrating handling of recovered 3D-printed device. (c) Image showing decellularized recovered 3D printed device. (d) Histological section of tissue recovered from device stained for C-peptide (green) and with 4,6-diamidino-2-phenylindole (DAPI; blue). We note that there are some red blood cells that autofluoresce green around the cellular cluster. Scale bar=25 μm. *p<0.01 (two-sided unpaired t-test when comparing large vs. smaller clusters, two-sided paried t-test when comparing 0 vs. 30 min).