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. Author manuscript; available in PMC: 2018 Jan 6.
Published in final edited form as: J Mol Biol. 2016 Nov 22;429(1):88–96. doi: 10.1016/j.jmb.2016.11.012

Figure 5. Reagent addition order influences the ssDNA binding activity of A3G.

Figure 5

(A) Representative EMSA showing the ssDNA binding activity of A3G in reactions in which Vif-derived peptides were first incubated 15 minutes with ssDNA and then A3G was added for an additional 15 minute incubation period, followed by native gel fractionation and imaging to detect free ssDNA (lower band) and ssDNA/A3G complexes (upper band).

(B) Representative EMSA showing the ssDNA binding activity of A3G in reactions in which Vif-derived peptides were first incubated 15 minutes with A3G and then ssDNA was added for an additional 15 minute incubation period, followed by native gel fractionation and imaging to detect free ssDNA (lower band) and ssDNA/A3G complexes (upper band).