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. Author manuscript; available in PMC: 2017 Jun 8.
Published in final edited form as: Cell Host Microbe. 2016 May 19;19(6):849–864. doi: 10.1016/j.chom.2016.05.001

Figure 4. Qsp1 Has Density-Dependent Impacts on Gene Expression and Cell Wall Integrity.

Figure 4

(A) Density-dependent RNA-seq. Expression profiling for qsp1Δ and wild-type log-phase and saturated cultures. The number of differentially expressed genes (padj < 0.05 by DESeq) is shown.

(B) Overlap of QSP1-regulated genes with published cell wall and secreted proteome (Eigenheer et al., 2007). QSP1-dependent genes are 2.3-fold enriched (p < 10−3 by hypergeometric distribution).

(C) Transmission electron microscopy.

(D and E) Quantification of cell wall width (D) and percentage of cells with visible cell wall layers (E). **p < 0.001, *p < 0.01 (t test). Error bars are SD.

(F) Stress assays. Cultures were incubated with stressors and plated for viability. The concentration where qsp1Δ viability is most different from wild-type is shown in red.

(G) LIV3-dependent genes. Expression profiling qsp1Δ and qsp1Δliv3Δ saturated cultures grown with or without synthetic Qsp1. Differentially expressed genes shown, with genes differentially expressed in qsp1Δ versus wild-type in yellow.

(H) LIV3 suppression of the qsp1Δ dry colony phenotype.