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. Author manuscript; available in PMC: 2017 Jun 8.
Published in final edited form as: Cell Host Microbe. 2016 May 19;19(6):849–864. doi: 10.1016/j.chom.2016.05.001

Figure 6. A Predicted Oligopeptide Transporter Is Required for Cells to Respond to Qsp1.

Figure 6

(A) Transcription factor binding sites near QSP1 and OPT1.

(B) Colony morphology.

(C) Capsule sizes. Cryptococcal capsule stained with India ink. >100 cells per genotype quantified. ***p < 10−5 (t test). Wild-type data from Figure 3 included for comparison. Error bars are SE.

(D) Stress assays. The concentration where qsp1Δ viability is most different from wild-type is shown in red. Wild-type data from Figure 4 included for comparison.

(E and F) Phagocytic index of unopsonized (E) and opsonized (F) C. neoformans. >1,200 BMDMs quantified per genotype. Wild-type data from Figure 2 included for comparison. Error bars are SD.

(G) Macrophage intracellular accumulation assay. CFUs isolated from BMDMs, normalized to starting inoculum. Wild-type data from Figure 2 included for comparison. Error bars are 95% confidence intervals. *p < 0.05 (bootstrap).

(H) Complementation assays.

(I) Stress assays as described in (D).