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. 2004 Oct 1;114(7):917–927. doi: 10.1172/JCI21484

Figure 7.

Figure 7

Normal expression of Pdx1, normal insulin contents and increased insulin secretion in βHT-IRS2 mice. (A) Immunohistochemical study of Pdx1 in control and βHT-IRS2 mice at 8 weeks (left panels) and at 12 weeks (right panels). Representative islets viewed on a computer monitor are shown. Original magnification ×100 (upper panels); ×200 (lower panels). (B) Taqman PCR analysis of Pdx1, Hnf1α, Hnf4α, and Foxo1 mRNA levels, normalized to β-actin mRNA levels in control (black bars) and βHT-IRS2 mice (white bars) at 8 weeks (left) and at 12 weeks (right). (C) Insulin secretion per islet (left) and insulin secretion normalized by cell number per islet (right) during static incubation of islets from control and βHT-IRS2 mice for 1 hour at the glucose levels indicated. Values are expressed as means ± SE (n = 5). Data were obtained in 3 independent experiments. (D) Insulin content per islet (left) and insulin content normalized by cell number per islet (right) in control and βHT-IRS2 mice. Values are expressed as means ± SE (n = 5). Data were obtained in 3 independent experiments. (E) Insulin secretion per islet during static incubation of islets from control and βHT-IRS2 mice for 1 hour at the glucose levels indicated with and without LY294002 (LY). Data were obtained in 3 independent experiments. *P < 0.05.