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Multiplex-PCR bands of samples. M: Molecular weight marker (100 bp), 1: Positive control of mix infection (500 bp, 327 bp and 204 bp), 2: Positive control of Dirofilaria repens (500 bp and 327 bp), 3: Positive control of Dirofilaria immitis (500 bp and 204 bp), 4: Only D. repens detected sample, 5, 6, 7: Only D. immitis detected samples, 8, 9, 10: Mix infected samples.
