Figure 2.

The three different Ig sequences identified in the database correspond to the prototypical teleost Ig isotyopes. Phylogenetic tree constructed by aligning the constant domains of the heavy chains of different Ig isotypes (IgM, IgD, and IgT) from different teleost species, including the ones identified in the gilthead sea bream database. The tree was constructed using the neighbor-joining method within the MEGA 6 package and bootstrapped 10,000 times. The GenBank accession numbers of each sequence can be found in parentheses after each species name.