Figure 7.

IgT expression can be affected by dietary conditions, which can have consequences on disease resistance. Normalized gene expression analysis of the different Igs in head kidney and posterior intestine of fish fed fish oil (FO)-based normal diets or diets with vegetable oils (VO) at 66% replacement (T5). Fish were tagged individually and kept unexposed (CTRL) or exposed by effluent with the parasite Enteromyxum leei. Infected fish were divided in two groups; the group EARLY had a well-established infection while the group LATE had an incipient infection. The gene expression was calculated using the β-actin as a reference gene, and the expression in the corresponding VO or FO CTRL groups (dotted line at y = 1) was used to calculate the fold changes. Values represent the mean + SE of n = 5–9. Asterisks (*) represent significant differences with the FO or VO CTRL group at p < 0.05.