Table 1.
Trial | Pathogen | Type of challenge | Sampling points | Fish weight (g)a | nia | Sampled tissues | Diet |
---|---|---|---|---|---|---|---|
T1 | Nodavirus strain 475-9/99 | Intramuscular injection | 24, 48 hpc | 45 | 30 | Head kidney | Basal |
Brain | |||||||
T2 | Photobacterium damselae subsp. piscidida | Bath challenge | 10 dpc | 20 | 100 | Head kidney | Basal + vaccine |
Spleen | |||||||
Intestine | |||||||
Serum | |||||||
Skin mucus | |||||||
T3 | Enteromyxum leei | Anal intubation | 17, 64 dpc | 60 | 36 | Head kidney | Basal |
Posterior intestine | |||||||
T4 | Enteromyxum leei | Effluent exposure | 133 dpc | 200 | 50 | Serum | Basal |
T5 | Enteromyxum leei | Effluent exposure | 102 dpc | 220 | 60 | Head kidney | Fish oil, vegetable oils |
Posterior intestine |
hpc, hours post-challenge; dpc, days post-challenge.
aFish weight and ni refer to the weight and number of fish at the beginning of the trial.