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. 2016 Dec 27;7:647. doi: 10.3389/fimmu.2016.00647

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Copyright © 2016 van Schaarenburg, Magro-Checa, Bakker, Teng, Bajema, Huizinga, Steup-Beekman and Trouw.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of stimulated cells from the C1q-deficient patient on the presence of C1q, (A) Western blot analysis of cell lysates from stimulated PBMCs in reducing conditions, (B) non-reducing and non-denaturing conditions. (C) Western blot analysis of the supernatant of the PBMCs from the patient and the healthy donor (control) after 72 h of culturing in reducing conditions. (D) In non-reducing and non-denatured conditions, the cell lysates and supernatant were added in the same amount. (E) A schematic representation of intact C1q and low molecular weight (LMW)-C1q. In LMW-C1q, positive charges are introduced in the collagen-like tail due the amino acid exchange Gly-Arg at the N-terminal region [modified from Ref. (2)].