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. 2016 Dec 27;7:654. doi: 10.3389/fphys.2016.00654

Figure 3.

Figure 3

Assessment of mitochondrial morphology regulators employing gene and protein expression. Liver and brain tissues were analyzed for gene (qRT-PCR) and protein expression (protein immunoblotting) of key mitochondrial morphology mediators. (A) Mitochondrial fission gene expression measured in liver. (B) Mitochondrial fission gene expression measured in brain. (C) Mitochondrial fusion gene expression measured in liver. (D) Mitochondrial fusion gene expression measured in brain. (E) Expression of mitochondrial fission proteins measured in liver. (F) Expression of mitochondrial fission proteins measured in brain. (G) Expression of mitochondrial fusion proteins measured in liver. (H) Expression of mitochondrial fusion proteins measured in brain. All gene expression data were collected using qRT-PCR with β-Actin as a loading control. All protein expression data were analyzed using β-Actin as a loading control. All data are presented as mean ± SEM (B6-Chow, B6-Ketogenic, BTBR-Chow, and BTBR-Ketogenic; n = 8 for each). *Indicates a difference due to diet at p < 0.05. †Indicates a difference due to genotype at p < 0.05.