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. 2016 Dec 27;7:654. doi: 10.3389/fphys.2016.00654

Figure 5.

Figure 5

Determination of mitochondrial gene and protein expression for mitochondrial quality control regulator BNIP3. Liver and brain tissues were analyzed for gene (qRT-PCR) and protein expression (protein immunoblotting) of the mitophagy promoter—BCL2/adenovirus E1B 19 kd-interacting protein 3 (BNIP3). (A) Gene expression of BNIP3 measured in liver. (B) Gene expression of BNIP3 measured in brain. (C) Protein expression of BNIP3 measured in liver. (D) Protein expression of BNIP3 measured in brain. All data were analyzed using β-Actin as a loading control and are presented as mean ± SEM (B6-Chow, B6-Ketogenic, BTBR-Chow, and BTBR-Ketogenic; n = 8 for each). *Indicates a difference due to diet at p < 0.05. †Indicates a difference due to genotype at p < 0.05.