Table 2.

Mitochondrial respirometry performed on mitochondria isolated from liver tissue homogenates using an XF^e24 Extracellular Flux Analyzer.

	B6		BTBR
	Chow	Ketogenic	Chow	Ketogenic
ADP	202±8.6*	139±12	165±24	150±16
Oligomycin	79±23*	37±8.3	64±10	56±9.3
FCCP	215±13*†	55±14	174±29	152±19
Antimycin A	19±10	17±5.1	41±15	18±9.2
Citrate synthase activity	34±1.0	35±2.6	34±1.4	36±2.1

Age-matched B6 and BTBR mice were sacrificed following 10–14 days of either chow or ketogenic diet feeding. Respiration buffer contained 5 mM succinate and 2 μM rotenone in order to isolate mitochondrial respiration through Complex II of the electron transport chain. Oxygen consumption rate was normalized to protein concentration, resulting in each value expressed as pmoles/min/μg mitochondrial protein. The mitochondrial enzyme citrate synthase is listed as a marker of mitochondrial abundance; each value is expressed as μM/min/mg protein. Data represent the mean ± SEM (B6-Chow, B6-Ketogenic, BTBR-Chow, and BTBR-Ketogenic; n = 11, 10, 15, and 10, respectively).

^*Indicates a difference due to diet at p < 0.05.

^†Indicates a difference due to genotype at p < 0.05.