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. 2016 Dec 27;7:1964. doi: 10.3389/fpls.2016.01964

FIGURE 1.

FIGURE 1

Silencing efficiency and specificity for target genes. Two-week-old seedlings were infiltrated with agrobacteria carrying different virus-induced gene silencing (VIGS) constructs and leaf samples were collected at 2 weeks after VIGS infiltration. (A) Silencing efficiency for target genes in VIGS-infiltrated plants. (B) Silencing specificity for target genes in VIGS-infiltrated plants. Transcript levels of the target genes were analyzed by qRT-PCR and data were normalized with a tomato actin gene. Silencing efficiency was calculated by comparing the transcript levels of each SlERF gene in corresponding pTRV2-SlERF-infiltrated plants to those in pTRV2-GUS-infiltrated plants. The transcript levels for each SlERF gene in the pTRV2-GUS-infiltrated plants were set as 100%. The transcript levels of each SlERF gene in VIGS-infiltrated plants were shown above the columns. Data are the mean ± SD from three independent experiments and different letters above the columns indicate significant difference at p < 0.05 level between pTRV2-SlERF- and pTRV2-GUS-infiltrated plants.