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. 2016 Dec 27;6:201. doi: 10.3389/fcimb.2016.00201

Figure 1.

Figure 1

Effect of whole S. aureus cells on HeLa cell cycle. (A) Comparison of cell cycle phases of asynchronized and synchronized HeLa cells after 25 h incubation (control experiment) reveal a comparable distribution (%) of cells in G1 (±60%), S (±17%), and G2/M (±23%) phases. (B) Effect of whole S. aureus cells on cell cycle progression: S. aureus USA300, USA300Δlpl and complemented mutant USA300Δlpl (pTX30-lpl). Twenty-five hours post-infection, cells were detached and together with adherent cells they were fixed in 70% ethanol overnight, stained with PI and analyzed by flow cytometry. Data were collected from 20,000 cells and analysis was performed with CFlow Accury6 software. The average percentage of cell cycle phase ± SD is indicated. The values of one representative experiment, out of four, is shown. P-values < 0.05 were considered to be significant.