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SHH signaling is reduced in the hmm−/− limb bud. In situ hybridization of Shh (A–D), Gli1 (E–H), Ptch1 (I–L), Ptch2 (M–P), Bmp2 (Q–T), and Gli3 (U–X) at St. 22/23. All images are oriented with the anterior side up and the posterior side down. (G–L) Yellow arrowheads indicate the weak expression of Gli1 and Ptch1. FLB, forelimb bud; HLB, hindlimb bud.
