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SHH protein is detectable by immunohistochemistry in the hmm−/− limb bud. Immunohistochemistry of SHH protein at the posterior limb bud of the St. 23 embryo is shown with fluorescent green. The nucleus is stained with DAPI. Frontal sections are oriented with the anterior side up and the posterior side down. (B,D) Higher magnification of the area enclosed by the dotted square in A and C. Scale bars, 50 μm (A,C), 10 μm (B,D).
