Skip to main content
. 2016 Dec 27;6:266. doi: 10.3389/fonc.2016.00266

Figure 3.

Figure 3

Choline kinase α (ChKα) as target for anticancer treatment. (A) ChKα converts free choline to phosphocholine (PC). (B) Knockdown of ChKα resulted in a dramatic reduction of cellular PC as evident by the decreased PC signal in high-resolution 1H MR spectrum obtained from water-soluble metabolites of MDA-MB-231 cells transfected with anti-ChKα siRNA compared to the spectrum from non-target siRNA control. (C) Stable knockdown of ChKα with a pSHAG-U6-shRNA vector significantly decreased MDA-MB-231 cell growth rate. Abbreviations: GPC, glycerophosphocholine. ***represents P < 0.001, average proliferation rates were calculated from five independent experiments. Adapted from Glunde et al. (69).