Figure 2.

Pharmacologic PARP inhibition does not increase ON axon regeneration or improve functional recovery from SCI. A, Mice underwent ON crush injury and were treated with veliparib or vehicle on d 3–17 postinjury. Representative images of ON from vehicle-treated and veliparib-treated mice. Multiple CTB-labeled axons proximal to the crush site are observed. Images are projections of confocal z-stacks through the entire ON. The eye is to the left, and the crush site is indicated by the blue arrow. Few axons extend centrally. B, High-magnification view of the lesion area (box) from A. C, Total number of regenerating ON fibers per mouse is presented as a function of distance central to the crush site. There is no statistically significant difference in the number of regenerating axons between the saline-treated group and the veliparib-treated group. Data are mean ± SE of n = 8 mice per group. D, Mice underwent midthoracic dorsal hemisection and then were treated with veliparib or vehicle on d 3–31 postinjury. The locomotor BMS score is plotted as a function of time after SCI. There is no statistically significant difference in the number of regenerating axons between the saline-treated group and the veliparib-treated group by one-way repeated-measure ANOVA. Data are mean ± SE for n = 8 mice per group.