Figure 1. TbTim17-RNAi affects presequence-containing and carrier proteins.

(a) Growth of the uninduced and induced TbTim17-RNAi cell line in SILAC medium. (lower) Immunoblot demonstrating the ablation of TbTim17 during TbTim17-RNAi when assayed in SILAC medium. EF1a is shown as a loading control. (b) The TbTim17-RNAi cell line was subjected to SILAC-based quantitative MS comparing protein abundances in induced versus uninduced cells. For proteins quantified in at least two of three independent biological replicates, the mean log₁₀ of normalized (norm.) ratios (induced/uninduced) was plotted against the −log₁₀ P value (two-sided t-test). A t-test significance level of 0.05 is indicated by the dashed horizontal line. Vertical dashed lines mark a fold-change in protein abundance of ±1.5. For a complete list of proteins, see Supplementary Data 1. (c) Relative (rel.) abundances of all proteins from the OM proteome, the preliminary cluster of IM proteins and predicted MTS-containing proteins that were detected by quantitative MS in TbTim17 RNAi experiments. (d) Individual abundance ratios (induced/uninduced) of all MCPs detected in the experiment shown in b. For MCP20, no P value (two-sided t-test) was assigned since the protein was only detected in two of the three biological replicates. Scale bar, s.d.; *, 0.05≥P≥0.01; **, 0.01≥P≥0.001; ***, 0.001≥P.