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. 2016 Dec 19;7:13866. doi: 10.1038/ncomms13866

Figure 2. HX-induced Sirt1 expression in white matter OPCs requires HIF1α.

Figure 2

(a) HIF1α stabilization of Sirt1 transcript expression in OPCs as revealed by representative RT–PCR represents higher level of Sirt1 mRNA in VHL cKO mice. GDPDH mRNA serves as a control. Mean±s.e.m., n=3 brains for each group. (b,c) Representative western blot demonstrates a transient increase of HIF1α expression in HX white matter at P18 with no significant effect at P11 (P=0.7955) and P45 (P=0.7333). Histograms show mean±s.e.m. (d,e) Graphs represent the percentages of Sirt1+Ki67+ and NG2+Ki67+ cells after HX white matter in WT and HIF1α KO mice. Number in parentheses within bar indicates number of samples (n=4 brains per group and per genotype; ****P<0.0001, one-way analysis of variance, Bonferroni post hoc test, mean±s.e.m.). (f,g) Western blot demonstrates no increase (P=0.8231) in Sirt1 and HIF1α expression in white matter of Hif1α KO mice. Actin was used as loading control (mean±s.e.m.; n=3 brains for each experiment, group and genotype).