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. 2016 Dec 19;7:13866. doi: 10.1038/ncomms13866

Figure 4. Sirt1 knockdown enhances OL differentiation in cultured white matter cells after neonatal HX.

Figure 4

NX and HX cells transfected with Sirt1 siRNA and scrambled control, and labelled with anti-Olig2 (a), -O4 (b) and -GalC (c) antibodies. Scale bar, 100 μm. Decreased percentages of Olig2+ (d), O4+ (e) and GalC+ cells (f) in control cultures after HX, and significantly increased OLs after Sirt1 siRNA (five NX and six HX brains per group; *P<0.05, one-way analysis of variance (ANOVA), Tukey's post hoc test, mean±s.e.m.). (g) Representative RT–PCR from NX and HX cells treated as above. Olig2, CNP and MBP mRNA expressions are reduced in controls and elevated in Sirt1 siRNA cultures. Actin serves as control (n=3 brains for each condition, each treatment). (h) Sirtinol increases OPC differentiation only in HX cultures. (i) Graph represents increase of GalC+ cells after sirtinol treatment (five NX and six HX brains for each treatment; *P<0.05, **P<0.01, one-way ANOVA, Tukey's post hoc test, mean±s.e.m.).