Figure 8. Sirt1 phosphorylation by Cdk2 requires sirtuin's deacetylase activity.

Confocal images from NX and HX white matter from WT and Cdk2 KO mice. Cells were immunolabelled with anti-Hif1α (a) and anti-pSirt1 Ser47 (c) antibodies. Graphs demonstrate percentages of Hif1α (b) and pSirt1 Ser47 (d) cells in HX white matter in both genotypes. Histograms show mean+s.e.m. Number in parentheses within bar indicates number of samples; four NX and six HX brains. (e) Representative western blot demonstrates lower expression of pSirt1 Ser47 in NX and HX conditions after sirtinol treatment. Actin was used as a loading control (n=3 brains for each condition, for each genotype).