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. 2016 Dec 21;7:13781. doi: 10.1038/ncomms13781

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Copyright © 2016, The Author(s)

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(a-c) qPCR analysis showing the expression of the YAP1 and Gp130 genes in Hep3B (a), P-HEPS (b) and DIH (c). qPCR data represent the mean (±s.e.m.) of three independent experiments; according to Student's t test. (d-f) Cells were treated with cycloheximide for the indicated amount of time prior to lysis. YAP1 and GP130 expression levels were determined by immunoblotting. A quantification is shown in the right panel; the amount of protein present in each of the untreated samples (normalized to TUBA or ACTB as loading controls) is set as 1.