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. 2016 Sep 23;5(4):33. doi: 10.3390/antibiotics5040033

Figure 1.

Figure 1

Muropeptide profile of S. carnosus TM300 by UPLC and UPLC/MS. Peptidoglycan was isolated, digested into muropeptides and analyzed by reversed phase UPLC and UPLC/MS. (a) Muropeptide profile of S. aureus SA113 Δspa obtained by UPLC. Muropeptide peaks that are identical with the ones of S. carnosus TM300 are numbered; (b) Muropeptide profile of S. carnosus TM300 as obtained by UPLC; (c) TIC of UPLC/MS analysis of S. carnosus TM300 peptidoglycan. Muropeptides of S. carnosus TM300 exhibit shorter retention times compared to S. aureus SA113 Δspa. UPLC/MS analysis revealed the presence of stem peptides containing only three amino acids instead of five. Masses of indicated peaks are shown in Table 1 including molecule composition and proposed molecular formula. Retention time of peaks in the UPLC (quaternary pumping system) was about 1 min longer than in the binary UPLC/MS system. Therefore, in Table 1 only TIC retention times are given (TIC: Total Ion current, recorded in positive mode).