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. 2016 Sep 23;5(4):33. doi: 10.3390/antibiotics5040033

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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UPLC/MS analysis of the PGN precursor. S. carnosus TM300 wild type and S. c. TM300 pPTX-hlyD-ftsEX were grown in B medium supplemented with 25 mM xylose. Precursors were concentrated, isolated and analyzed by UPLC/MS. Peaks highlighted by arrows were identified to harbor the same masses including the mass for the classical precursor UDP-MurNAc-pentapeptide (1149.37 g/mol). Masses for UDP-MurNAc-tripeptide (1007.2774 g/mol) or UDP-MurNAc-tetrapeptide (1078.3145 g/mol) were not detected in any of the analyses.