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. 2016 Sep 23;5(4):33. doi: 10.3390/antibiotics5040033

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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The ΔccpA mutant showed a decrease in colony size and a reduction in growth rate. (A) On solid media colonies, the deletion mutant appeared clearly smaller than the parental strains. The glucose concentration seemed to have only a minor influence on colony size; (B) In liquid media, S. carnosusTM300 ΔccpA also showed a growth defect compared to the wild type strain. In both cases, the effect could be complemented. The influence of the glucose concentration in both experiments was negligible. (Bar size: 2 mm; Glc: glucose).