Table 1.
LC-MS/MS parameters of selected compounds and amount of three Salvia species (µg/kg).
| No | Analyses | RT a | Parent Ion (m/z) b | Ionization Mode | r2 c | RSD (%) d | Linearity Range (µg/L) | LOD / LOQ (µg/L) e | Recovery (%) | U f | S. brachyantha | S. aethiopis | S. microstegia |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Quinic acid | 3.32 | 190.95 | Neg | 0.9927 | 0.0388 | 250–10,000 | 22.3 / 74.5 | 103.3 | 4.8 | 161.26 | 224.58 | 48.8 |
| 2 | Malic acid | 3.54 | 133.05 | Neg | 0.9975 | 0.1214 | 250–10,000 | 19.2 / 64.1 | 101.4 | 5.3 | 71.67 | 0 | 0 |
| 3 | trans-Aconitic acid | 4.13 | 172.85 | Neg | 0.9933 | 0.3908 | 250–10,000 | 15.6 / 51.9 | 102.8 | 4.9 | ND | ND | ND |
| 4 | Gallic acid | 4.29 | 169.05 | Neg | 0.9901 | 0.4734 | 25–1000 | 4.8 / 15.9 | 102.3 | 5.1 | ND | ND | ND |
| 5 | Chlorogenic acid | 5.43 | 353 | Neg | 0.9932 | 0.1882 | 250–10,000 | 7.3 / 24.3 | 99.7 | 4.9 | 355.05 | 25.42 | 0 |
| 6 | Protocatechuic acid | 5.63 | 152.95 | Neg | 0.9991 | 0.5958 | 100–4000 | 25.8 / 85.9 | 100.2 | 5.1 | 31.39 | 88.24 | 57.04 |
| 7 | Tannic acid | 6.46 | 182.95 | Neg | 0.9955 | 0.9075 | 100–4000 | 10.2 / 34.2 | 97.8 | 5.1 | ND | ND | ND |
| 8 | trans-Caffeic acid | 7.37 | 178.95 | Neg | 0.9942 | 1.0080 | 25–1000 | 4.4 / 14.7 | 98.6 | 5.2 | 151.77 | 289.68 | 176.8 |
| 9 | Vanillin | 8.77 | 151.05 | Neg | 0.9995 | 0.4094 | 250–10,000 | 10.1 / 33.7 | 99.2 | 4.9 | 40.75 | 52.28 | 28.91 |
| 10 | p-Coumaric acid | 9.53 | 162.95 | Neg | 0.9909 | 1.1358 | 100–4000 | 15.2 / 50.8 | 98.4 | 5.1 | 260.97 | 486.72 | 437.15 |
| 11 | Rosmarinic acid | 9.57 | 358.9 | Neg | 0.9992 | 0.5220 | 250–10,000 | 10.4 / 34.8 | 101.7 | 4.9 | 7619.58 | 1904.53 | 173.62 |
| 12 | Hesperidin | 9.69 | 611.1 | Poz | 0.9973 | 0.1363 | 250–10,000 | 21.6 / 71.9 | 100.2 | 4.9 | 354.74 | ND | ND |
| 13 | Rutin | 10.18 | 609.1 | Neg | 0.9971 | 0.8146 | 250–10,000 | 17.0 / 56.6 | 102.2 | 5.0 | 399.43 | ND | ND |
| 14 | Hyperoside | 10.43 | 463.1 | Neg | 0.9549 | 0.2135 | 100–4000 | 12.4 / 41.4 | 98.5 | 4.9 | 30.85 | 71.43 | 23.38 |
| 15 | 4-Hydroxybenzoic acid | 11.72 | 136.95 | Neg | 0.9925 | 1.4013 | 25–1000 | 3.0 / 10.0 | 106.2 | 5.2 | 23.37 | 29.17 | 11.45 |
| 16 | Salicylic acid | 11.72 | 136.95 | Neg | 0.9904 | 0.6619 | 25–1000 | 4 / 13.3 | 106.2 | 5.0 | 19.96 | 26.33 | 11.08 |
| 17 | Myricetin | 11.94 | 317 | Neg | 0.9991 | 2.8247 | 100–4000 | 9.9 / 32.9 | 106.0 | 5.9 | ND | ND | ND |
| 18 | Fisetin | 12.61 | 284.95 | Neg | 0.9988 | 2.4262 | 100–4000 | 10.7 / 35.6 | 96.9 | 5.5 | ND | ND | ND |
| 19 | Coumarin | 12.52 | 146.95 | Poz | 0.9924 | 0.4203 | 100–4000 | 9.1 / 30.4 | 104.4 | 4.9 | ND | ND | ND |
| 20 | Quercetin | 14.48 | 300.9 | Neg | 0.9995 | 4.3149 | 25–1000 | 2.0 / 6.8 | 98.9 | 7.1 | ND | ND | ND |
| 21 | Naringenin | 14.66 | 270.95 | Neg | 0.9956 | 2.0200 | 25–1000 | 2.6 / 8.8 | 97.0 | 5.5 | ND | 28.91 | 22.55 |
| 22 | Hesperetin | 15.29 | 300.95 | Neg | 0.9961 | 1.0164 | 25–1000 | 3.3/ 11.0 | 102.4 | 5.3 | ND | 11.42 | 4.05 |
| 23 | Luteolin | 15.43 | 284.95 | Neg | 0.9992 | 3.9487 | 25–1000 | 5.8 / 19.4 | 105.4 | 6.9 | 25.41 | 171.50 | 437.46 |
| 24 | Kaempferol | 15.43 | 284.95 | Neg | 0.9917 | 0.5885 | 25–1000 | 2.0 / 6.6 | 99.1 | 5.2 | 129.92 | 764.82 | 1954.28 |
| 25 | Apigenin | 17.31 | 268.95 | Neg | 0.9954 | 0.6782 | 25–1000 | 0.1 / 0.3 | 98.9 | 5.3 | 222.54 | 416.76 | 1207.27 |
| 26 | Rhamnetin | 18.94 | 314.95 | Neg | 0.9994 | 2.5678 | 25–1000 | 0.2 / 0.7 | 100.8 | 6.1 | 2.50 | 25.19 | 12.89 |
| 27 | Chrysin | 21.18 | 253 | Neg | 0.9965 | 1.5530 | 25–1000 | 0.05 / 0.17 | 102.2 | 5.3 | 0.94 | 0.42 | 2.51 |
a RT: retention time; b Parent ion (m/z): Molecular ions of the standard compounds (mass to charge ratio); c r2: coefficient of determination; d RSD: relative standard deviation; e LOD/LOQ (µg/L): limit of detection/limit of quantification; f U (%): percent relative uncertainty at 95% confidence level (k = 2); ND: not determined.