Figure 2. Twofold screw xylan predominates in wild type but is barely detectable in the cellulose-deficient mutant irx3.

An overlay is shown of carbohydrate regions of refocussed CP-INADEQUATE ¹³C NMR spectra of wild-type and irx3. Red and green labelling illustrates the two distinct xylan conformations shown clearly here by the distinct Xn4 and Xn5 shifts. The spectra were recorded as in Fig. 1, using a total spin-echo duration of 2.2 ms.