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. 2016 Dec 1;113(51):14710–14715. doi: 10.1073/pnas.1604572113

Fig. S4.

Fig. S4.

NADP-dependent IDH1 and IDH2 in hfRPE regulates NAD/NADH, mitochondrial bioenergetics, and fatty acid synthesis. (A–C) hfRPE cells were transfected with siGENOME human IDH1 and IDH2 siRNA on the initial and third day after plating. Cells transfected with nontarget siRNA were used as controls. The silencing efficiency was (A) determined by Western blot analysis of IDH1 and IDH2 expression and (B and C) quantified. Data are mean ± SD (n = 3). *P < 0.05 vs. SiCon (siRNA control). (D) Knockdown of IDH1 or IDH2 increases NADH in hfRPE. (Data are normalized to the control value in D–F.) (E) Inhibition of NADP-dependent IDHs by 5 mM OMA increases NAD/NADH (n = 3). Con, control. (F) Knockdown of either IDH1 or IDH2 increases the ADP/ATP ratio relative to control (n = 3). (G) Enrichment of palmitate after knockdown of IDH1 or IDH2 in hfRPE cells (n = 3). Mean ± SD (n = 3). *P < 0.05 vs. Con or SiCon (nontarget siRNA control).