Fig. S4.
The p.D387N allele is loss of function for the response to IL-17E/IL-25. PBMCs from three controls, P5, P6, and two healthy relatives were cultured with thymic stromal lymphopoietin for 24 h, harvested, and stimulated with IL-2 and -17E/IL-25 for an additional 72 h. IL-5 was determined in the culture supernatants by ELISA. Error bars represent the SD. NS, not stimulated.