The inhibitory effect of MIA-602 on the viability of GC cells in vitro. (A–C) The viability of KATO-III (A), SGC-7901 (B), and SK-GT5 (C) cells treated with MIA-602 (0.1, 1, 5, or 10 μM) or vehicle solution for 48 h was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. (D–F) Colony formation of KATO-III (D), SGC-7901 (E), and SK-GT5 (F) cells was decreased by treatment with MIA-602 (filled bar) compared with vehicle treatment (open bar). Quantitative analysis of colony numbers is given on the right. Error bars indicate SEM. *P < 0.05, **P < 0.01, ***P < 0.001 by a one-way ANOVA with post hoc intergroup comparisons (A–C) or Student’s t test (D–F); n = 3 in each group (A–F).