Fig. 2.

The results of one experiment with resealed human red-blood-cell ghosts that have been centrifuged onto a glass coverslip coated with PDL to enhance attachment. The ghosts were first washed and sphered in hypotonic solution B. A portion of the ghosts was then layered onto the top of a slightly more dense solution C and then centrifuged as detailed in the text. The coverslip was then transferred to a small chamber for microscopic observation. (A) The sphered ghosts adhering to the coverslip. (B) The transformation of the shape of the ghosts from spheres to biconcave discs when the chamber was flushed with isotonic solution A. It is important to note that the orientation of the biconcave ghosts on the coverslip was flat. (Scale bar in A, which also applies to B and to Figs. 3 and 4: 10 μm.) See text for discussion.