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. 2016 Dec 7;113(51):E8335–E8343. doi: 10.1073/pnas.1610670114

Fig. 5.

Fig. 5.

HY5 binds to EMF and influences TMP. (A) Six kinds of element were predicted in the EMF. (B) Yeast one-hybrid assay tested the ability of HY5, HYH, and GT-1 to bind to C/A-box (pLac-CA), GAAAA(pLac-GA), mutational C/A-box (pLac-▵CA), or GAAAA(pLac-▵GA). The blue plaque indicates positive interaction between the tested proteins and corresponding elements. (C) The enrichment of regions 1, 2, 3 (as shown in Fig. 3A), and 5 (EMF) in P5CS1 precipitated by HY5 antibody in Col-0 and hy5 hyh. (D) The transcript levels of P5CS1 and (E) proline content at the Ctr, Sp0, R2, and Sp2 stage in wild-type and mutant plants. Values between bars denote fold-change. (F) H3K4me3 levels of P5CS1 at region 3 (Fig. 3A) at the Ctr, Sp0, R2, and Sp2 stage in hy5 hyh double mutants. C–F were repeated three times and a representative result is shown as mean ± SEM (n = 3). Asterisks in C indicate statistical significance between hy5 hyh and Col-0 based on Student’s t test. **P < 0.01; *P < 0.05.