Fig. 1.

Characterization of IEC-Cosmc mice. (A) Schematic of O-glycan biosynthesis: Cosmc is the chaperone for the T-synthase and required to extend O-glycans beyond the Tn antigen. GPs, glycoproteins. Purified IECs from CR and SI from KO, mosaic, and gender-matched WT mice were probed with anti-Cosmc and anti-Actin antibodies by Western blot (B) and assayed for T-synthase and mannosidase activities (2 mice pooled per group; 2 groups evaluated/tissue per genotype; age 2–3 mo) (C). (D) Tn expression was analyzed by IHC on formalin fixed colorectum (CR) and ileum (SI) from KO, mosaic, and WT mice (n = 3–5 mice per group, 3 mo old). (Scale bars: Left and Center, 200 μM; Right, 500 μM.) (E) UEA-I lectin was used to analyze α1,2-fucose expression in the colorectum and ileum from KO, mosaic, and WT mice; Insets show UEA-I preincubated with 100 mM free fucose denoted as “+Fuc” (n = 3–5 mice per group, 3 mo old). Representative images shown. (Scale bars: Top, 1 mm; Bottom, 500 μM.)