Fig. 3.

DSS colitis in KO and mosaic mice. KO (A–D), mosaic (E–G), and WT mice (A–G) were treated with 2.5% DSS for 4 d and then switched to drinking water. Body weight (A and E), disease activity index (% weight change, stool softness, blood in stool) (B and F), survival (Mantel–Cox test) (C), histology (D), and fecal lipocalin-2 (G) were measured. n = 9 mice per group, age 3 mo (A–C); n = 6 KO, 9 WT, killed on day 8 and the percent normal, eroded, and ulcerated epithelia on H&E-stained CR was quantified (D); n = 5–6 mice per group, age 3 mo (E–G): repeated two independent times, one representative experiment shown (E and F) or pooled data (G); *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001 from ANOVA with Sidak’s post hoc test for multiple comparisons (A, B, and D–F) or Mann–Whitney (G); 0.05 < P < 0.1 listed; mean ± SE (A, B, and D–G).