Fig. 8.

c-Src knockdown restores organoid formation by Cbl triple-deficient MECs. (A) Adeno-Cre infected TMut MECs were stained with ProteoStat and antibodies against phosphorylated c-Src (Left) or phosphorylated EGFR (Right). Because Cre adenovirus is tagged with GFP, nuclei show green fluorescence. Arrowheads indicate colocalization of p-Src and aggregates. (Scale bars, 5 μm.) (B) TMut MECs were infected with lentivirus encoding tetracycline-inducible shRNA against c-Src (shRNA #1, #2, and #3) or nontargeting control followed by Cre adenovirus and subjected to organoid formation assay in Matrigel in the presence or absence of 1 μg/mL doxycycline. Organoids were enumerated 7 d later. An asterisk indicates no organoid. A representative result from two independent experiments is shown. (C) Proposed mechanism of cell death induced in the absence of Cbl family proteins.