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. 2016 Dec 5;113(51):E8228–E8237. doi: 10.1073/pnas.1615677113

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Fig. S1. — Mammary glands develop normally in the absence of Cbl-b and Cbl-c. (A) Whole-mount staining of #4 mammary gland from 10-wk-old virgin WT and homozygous Cbl^flox;Cblb^del;Cblc^del (TMut) female mice. (Scale bars, 2 mm.) (B) Surface marker analysis of MECs from 10-wk-old WT and TMut female mice. After magnetic depletion of cells expressing nonepithelial lineage markers (CD45, CD31, TER119, and CD140α), MECs were stained for CD29, CD24, and Sca-1. Left panels of each genotype show CD29 and CD24 expression patterns of cells electronically gated for the absence of nonepithelial lineage markers (CD45, CD31, TER119, and CD140α). Right panels show CD24 and Sca-1 expression patterns of CD29^medCD24^hi population marked in the Left panels. Numbers represent mean ± SEM of the percentage of each population out of respective parent populations from three mice.