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Acute Cbl deletion on the Cbl-b, Cbl-c–deficient background does not confer proliferative advantage to bone marrow cells. WT control and TMut mice were crossed to mice carrying the Cre activity reporter Tg(CAG-cat,-EGFP)39Miya (53) and the tamoxifen-inducible Cre Gt(ROSA)26Sor <tm1(cre/ERT2)Tyj> (54). Bone marrow cells were isolated, cultured in the presence (solid lines) or absence (dotted lines) of 10 nM 4-hydroxytamoxifen, and GFP expression was analyzed by flow cytometry.
