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. 2016 Dec 5;113(51):14864–14869. doi: 10.1073/pnas.1607695114

Fig. S9.

Fig. S9.

Specificity of the anti-PSBS antibodies assayed by peptide competition. Immunoblot analysis of protein extracts from WT cells treated with UV-B for 6 h (+UV) to induce the expression of endogenous PSBS or left untreated (−UV). The blots were probed with anti-PSBS antibodies only (−) or in competition with the peptide used for immunization (Pep 93: C+AINEGSGKFVDEESA, corresponding to CrPSBS231–245) or of a control peptide (Pep 94: C+DTISERPAGPLQDPR, corresponding to CrPSBS140–154) (both 7 μg⋅mL−1). Pep 93, but not the Pep 94 control, efficiently prevents binding of the antibodies to PSBS.