Fig. 1.

Sequence alignment of bacterial trigger factors. The sequences of trigger factors from Bacillus subtilis, Haemophilus influenzae, Mycoplasma genitalium, E. coli, and V. cholerae were aligned by using clustalw (43). Invariant residues are indicated with red letters. Secondary structures are shown below the sequence: α-helices are drawn as cylinders, β-strands are drawn as arrows, other elements appear as solid lines, and structurally unobserved residues in the midsequence and at the C terminus are shown as dashed lines. Elements in domain I are blue, elements in domain II are green, and elements in domain III are red.